|
Eleven cases of orthotopic cardiac transplantations were performed using 33 mongrel dogs. The main causes of death were pulmonary edema and bleeding at aanstomotic site. To compare the efficacy of cardiac preservation, purine metabolites(ATP, ADP, AMP) were examed during 24 hour of cold storgage (0℃) of the mongrel dog hearts after using two different yeps of cardioplegic solutions. The hypothermic arrest with total cardiopulmonary bypass method was empolyed in St. thomas solution preservation cases Left ventricular myocardial specimens were obtained after 0.5, 2, 4, 8, 12, and 24 hours at 0℃, and stored at -75℃deep freezer until analysis. Specimens were analyzed for levels of adenine nucleotides by high performance liquid chromatography. The ATP content in the UWS(University of Wisconsin solution) group tends to be higher than that of the combined hypothermic arrest STS9St. Thomas) group after 2, 4, 8 and 12 hours of preservation respectively. the ADP content in the UWS group was higher than that of the STS group at 4, 8, 12, and 24 hours. the AMP contents did not change significantly in the two groups. These results strongly suggests that although UW solution did not provide satisfactory long-term preservatoin of heart, UWA group is better than STS group with hypothermic arrest method.
|